Wednesday, October 2, 2019

Transgenic Rice Plants Essay -- essays research papers

  Ã‚  Ã‚  Ã‚  Ã‚  For centuries, rice has been one of the most important staple crops for the world and it now currently feeds more than two billion people, mostly living in developing countries. Rice is the major food source of Japan and China and it enjoys a long history of use in both cultures. In 1994, worldwide rice production peaked at 530 million metric tons. Yet, more than 200 million tons of rice are lost each year to biotic stresses such as disease and insect infestation. This extreme loss of crop is estimated to cost at least several billion dollars per year and heavy losses often leave third world countries desperate for their staple food. Therefore, measures must be taken to decrease the amount of crop loss and increase yields that could be used to feed the populations of the world. One method to increase rice crop yields is the institution of transgenic rice plants that express insect resistance genes. The two major ways to accomplish insect resistance in rice are the introduction of the potato proteinas e inhibitor II gene or the introduction of the Bacillus thuringiensis toxin gene into the plant's genome. Other experimental methods of instituting insect resistance include the use of the arcelin gene, the snowdrop lectin/GNA (galanthus nivallis agglutinin) protein, and phloem specific promoters and finally the SBTI gene.   Ã‚  Ã‚  Ã‚  Ã‚  The introduction of the potato proteinase inhibitor II gene, or PINII, marks the first time that useful genes were successfully transferred from a dicotyledonus plant to a monocotyledonous plant. Whenever the plant is wounded by insects, the PINII gene produces a protein that interferes with the insect's digestive processes. These protein inhibitors can be detrimental to the growth and development of a wide range of insects that attack rice plants and result in insects eating less of the plant material. Proteinase inhibitors are of particular interest because they are part of the rice plant's natural defense system against insects. They are also beneficial because they are inactivated by cooking and therefore pose no environmental or health hazards to the human consumption of PINII treated rice.   Ã‚  Ã‚  Ã‚  Ã‚  In order to produce fertile transgenic rice plants, plasmid pTW was used, coupled with the pin 2 promoter and the inserted rice actin intron, act 1. The combi... ... SBTI gene is being cloned into vectors and is being used to transform other types of embryos using the particle gun technique.   Ã‚  Ã‚  Ã‚  Ã‚  In conclusion, through the use of new technologies such as the introduction of potato proteinase inhibitor II gene, the establishment of the Bacillus thuringiensis toxin gene and the experimental methods of using the arcelin gene, the snowdrop lectin/GNA (galanthus nivallis agglutinin) protein, and phloem specific promoters and finally the SBTI gene, rice plants have become almost completely resistant to insects that used to destroy much of the crop. This has been an important step in biotechnology because the improvement of rice plants is a major concern that could potentially effect almost all of the populations of the world. Biotechnology has become an increasingly accepted method of solving some of the major problems in agriculture, medicine, and industry. Potentially, with the advancements of many techniques, almost whenever people eat, drink, take medicine, or go to work, they will be touched in some way by the many complicated processes of biotechnology, that are striving to make our world a better place to exist in.

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